Microsatellite genotyping of Plasmodium vivax infections and their relapses in pregnant and non-pregnant patients on the Thai-Myanmar border
Identifieur interne : 000189 ( France/Analysis ); précédent : 000188; suivant : 000190Microsatellite genotyping of Plasmodium vivax infections and their relapses in pregnant and non-pregnant patients on the Thai-Myanmar border
Auteurs : Supinya Thanapongpichat [Thaïlande] ; Rose Mcgready [Thaïlande] ; Christine Luxemburger [France] ; Nicholas Day [Royaume-Uni] ; Nicholas White [Royaume-Uni] ; Francois Nosten [Thaïlande] ; Georges Snounou [France] ; Mallika Imwong [Thaïlande]Source :
- Malaria Journal [ 1475-2875 ] ; 2013.
Abstract
Background
Plasmodium vivax infections in pregnancy are associated with low birth weight and anaemia. This parasites species is also characterised by relapses, erythrocytic infections initiated by the activation of the dormant liver stages, the hypnozoites, to mature. Genotyping of P. vivax using microsatellite markers has opened the way to comparative investigations of parasite populations. The aim of the study was to assess whether there were any differences between the parasites found in pregnant and non-pregnant patients, and/or between the admission infections and recurrent episodes during follow-up.
Methods
Blood samples were collected from 18 pregnant and 18 non-pregnant patients, who had at least two recurrent episodes during follow-up, that were recruited in two previous trials on the efficacy of chloroquine treatment of P. vivax infections on the Thai-Myanmar border. DNA was purified and the P. vivax populations genotyped with respect to eight polymorphic microsatellite markers. Analyses of the genetic diversity, multiplicity of infection (MOI), and a comparison of the genotypes in the samples from each patient were conducted.
Results
The P. vivax parasites present in the samples exhibited high genetic diversity (6 to 15 distinct allelic variants found for the 8 loci). Similar expected heterozygosity (H e) values were obtained for isolates from pregnant (0.837) and non-pregnant patients (0.852). There were modest differences between the MOI values calculated for both admission and recurrence samples from the pregnant patients (2.00 and 2.05, respectively) and the equivalent samples from the non-pregnant patients (1.67 and 1.64, respectively). Furthermore, the mean number of distinct alleles enumerated in the admission samples from the pregnant (6.88) and non-pregnant (7.63) patients were significantly lower than that found in the corresponding recurrent episodes samples (9.25 and 9.63, respectively).
Conclusions
The P. vivax populations circulating in inhabitants along the Thai-Myanmar border, an area of low malaria transmission, displayed high genetic diversity. A subtle increase in the multiplicity of P. vivax infections in pregnant patients suggests a higher susceptibility to infection. The higher allelic diversity in the relapse as compared to the admission samples in both patient groups is consistent with the hypothesis that a febrile episode promotes the activation of hypnozoites.
Url:
DOI: 10.1186/1475-2875-12-275
Affiliations:
Links toward previous steps (curation, corpus...)
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Hal:inserm-00851404Le document en format XML
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<country key="TH"></country> </address> <ref type="url">http://www.tropmedres.ac/home</ref> </desc> <listRelation> <relation active="#struct-302612" type="direct"></relation> <relation active="#struct-303866" type="direct"></relation> <relation active="#struct-304717" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-302612" type="indirect"><org type="institution" xml:id="struct-302612" status="VALID"> <orgName>University of Oxford [Oxford]</orgName> <desc> <address> <addrLine>Wellington Square, Oxford OX1 2JD</addrLine> <country key="GB"></country> </address> <ref type="url">http://www.ox.ac.uk/</ref> </desc> </org></tutelle><tutelle active="#struct-304717" type="indirect"><org type="institution" xml:id="struct-304717" status="VALID"> <orgName>Wellcome Trust</orgName> <desc> <address> <country key="GB"></country> </address> <ref type="url">https://wellcome.ac.uk/about-us</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Thaïlande</country></affiliation></author><author><name sortKey="Snounou, Georges" sort="Snounou, Georges" uniqKey="Snounou G" first="Georges" last="Snounou">Georges Snounou</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-81476" status="OLD"> <orgName>Immunité et Infection</orgName> <desc> <address> <country key="FR"></country> </address> </desc> <listRelation> <relation active="#struct-93591" type="direct"></relation> <relation active="#struct-300630" type="direct"></relation> <relation name="U945" active="#struct-303623" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-93591" type="direct"><org type="institution" xml:id="struct-93591" status="OLD"> <orgName>Université Pierre et Marie Curie - Paris 6</orgName> <orgName type="acronym">UPMC</orgName> <date type="end">2017-12-31</date> <desc> <address> <addrLine>4 place Jussieu - 75005 Paris</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.upmc.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-300630" type="direct"><org type="institution" xml:id="struct-300630" status="VALID"> <orgName>IFR113</orgName> <desc> <address> <country key="FR"></country> </address> </desc> </org></tutelle><tutelle name="U945" active="#struct-303623" type="direct"><org type="institution" xml:id="struct-303623" status="VALID"> <idno type="IdRef">026388278</idno> <orgName>Institut National de la Santé et de la Recherche Médicale</orgName> <orgName type="acronym">INSERM</orgName> <desc> <address> <addrLine>101, rue de Tolbiac, 75013 Paris </addrLine> <country key="FR"></country> </address> <ref type="url">http://www.inserm.fr</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Imwong, Mallika" sort="Imwong, Mallika" uniqKey="Imwong M" first="Mallika" last="Imwong">Mallika Imwong</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-208050" status="INCOMING"> <orgName>Department of Molecular Tropical Medicine and Genetics</orgName> <desc> <address> <addrLine>Faculty of Tropical Medicine, Bangkok</addrLine> <country key="TH"></country> </address> </desc> <listRelation> <relation active="#struct-303866" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-303866" type="direct"><org type="institution" xml:id="struct-303866" status="VALID"> <orgName>Mahidol University [Bangkok]</orgName> <desc> <address> <addrLine>999 Phuttamonthon 4 Road, Salaya, Nakhon Pathom 73170 Thailand</addrLine> <country key="TH"></country> </address> <ref type="url">https://mahidol.ac.th/</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Thaïlande</country></affiliation></author></analytic><idno type="DOI">10.1186/1475-2875-12-275</idno><series><title level="j">Malaria Journal</title><idno type="ISSN">1475-2875</idno><imprint><date type="datePub">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="it"><term>Genetic diversity</term><term>Malaria</term><term>Plasmodium vivax</term><term>Pregnancy</term><term>Relapse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Background
Plasmodium vivax infections in pregnancy are associated with low birth weight and anaemia. This parasites species is also characterised by relapses, erythrocytic infections initiated by the activation of the dormant liver stages, the hypnozoites, to mature. Genotyping of P. vivax using microsatellite markers has opened the way to comparative investigations of parasite populations. The aim of the study was to assess whether there were any differences between the parasites found in pregnant and non-pregnant patients, and/or between the admission infections and recurrent episodes during follow-up.
Methods
Blood samples were collected from 18 pregnant and 18 non-pregnant patients, who had at least two recurrent episodes during follow-up, that were recruited in two previous trials on the efficacy of chloroquine treatment of P. vivax infections on the Thai-Myanmar border. DNA was purified and the P. vivax populations genotyped with respect to eight polymorphic microsatellite markers. Analyses of the genetic diversity, multiplicity of infection (MOI), and a comparison of the genotypes in the samples from each patient were conducted.
Results
The P. vivax parasites present in the samples exhibited high genetic diversity (6 to 15 distinct allelic variants found for the 8 loci). Similar expected heterozygosity (H e) values were obtained for isolates from pregnant (0.837) and non-pregnant patients (0.852). There were modest differences between the MOI values calculated for both admission and recurrence samples from the pregnant patients (2.00 and 2.05, respectively) and the equivalent samples from the non-pregnant patients (1.67 and 1.64, respectively). Furthermore, the mean number of distinct alleles enumerated in the admission samples from the pregnant (6.88) and non-pregnant (7.63) patients were significantly lower than that found in the corresponding recurrent episodes samples (9.25 and 9.63, respectively).
Conclusions
The P. vivax populations circulating in inhabitants along the Thai-Myanmar border, an area of low malaria transmission, displayed high genetic diversity. A subtle increase in the multiplicity of P. vivax infections in pregnant patients suggests a higher susceptibility to infection. The higher allelic diversity in the relapse as compared to the admission samples in both patient groups is consistent with the hypothesis that a febrile episode promotes the activation of hypnozoites.</p> </div></front></TEI><affiliations><list><country><li>France</li><li>Royaume-Uni</li><li>Thaïlande</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Thanapongpichat, Supinya" sort="Thanapongpichat, Supinya" uniqKey="Thanapongpichat S" first="Supinya" last="Thanapongpichat">Supinya Thanapongpichat</name></noRegion><name sortKey="Imwong, Mallika" sort="Imwong, Mallika" uniqKey="Imwong M" first="Mallika" last="Imwong">Mallika Imwong</name><name sortKey="Mcgready, Rose" sort="Mcgready, Rose" uniqKey="Mcgready R" first="Rose" last="Mcgready">Rose Mcgready</name><name sortKey="Nosten, Francois" sort="Nosten, Francois" uniqKey="Nosten F" first="Francois" last="Nosten">Francois Nosten</name></country><country name="France"><noRegion><name sortKey="Luxemburger, Christine" sort="Luxemburger, Christine" uniqKey="Luxemburger C" first="Christine" last="Luxemburger">Christine Luxemburger</name></noRegion><name sortKey="Snounou, Georges" sort="Snounou, Georges" uniqKey="Snounou G" first="Georges" last="Snounou">Georges Snounou</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Day, Nicholas" sort="Day, Nicholas" uniqKey="Day N" first="Nicholas" last="Day">Nicholas Day</name></noRegion><name sortKey="White, Nicholas" sort="White, Nicholas" uniqKey="White N" first="Nicholas" last="White">Nicholas White</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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